Industry Knowledge
Features of Agilent Compatible Tips
2. No DNase, RNase, no pyrogen;
3. High transparency, super hydrophobicity, smooth surface, no hanging liquid;
4. Good temperature resistance, no deformation under high temperature and high pressure;
5. Pass strict air tightness inspection to ensure that the product does not leak;
6. High-quality filter element with good sealing performance to prevent cross-infection;
7. Good verticality and air tightness, strong adaptability;
8. Ensure the accuracy and precision in the pipetting process and ensure the consistency of the experimental results;
9. Independent identification, each package has an independent item number identification, which is easy to track and trace.
How to Inject Agilent Compatible Tips Without Traces?
1.
Agilent compatible tips column temperature fluctuation. (Even small temperature changes can cause fluctuations in the baseline. Typically affect differential, conductivity, less sensitive UV or other photoelectric type detectors.)
2. Control the temperature of the column and mobile phase, and use a heat exchanger before the detector. Figure 2. The mobile phase is not uniform. (Baseline drift caused by changes in mobile phase conditions is greater than that caused by temperature.)
3. Use HPLC-grade solvents, high-purity salts and additives. The mobile phase was degassed before use, and helium was used during use. The flow cell is contaminated or gassy Flush the flow cell with methanol or other strong polar solvent. 1N nitric acid can be used if necessary. (do not use hydrochloric acid)
4. The outlet of the detector is blocked. (High pressure causes flow cell window to rupture, creating noisy baseline) Remove blockage or replace tubing. Refer to the detector manual to replace the flow cell window.
5. Improper ratio of mobile phase or change of flow rate Change the ratio or flow rate. To avoid this problem, periodically check the mobile phase composition and flow rate.
6. The column balance is slow, especially when the mobile phase changes, use a medium-strength solvent to flush. When changing the mobile phase, use 10-20 times the volume of the new mobile phase column to flush before analysis.
7. The mobile phase is contaminated, deteriorated or made of low-quality solvents, check the composition of the mobile phase. Strongly retained substances (high K' values) in samples using high-quality chemical reagents and HPLC-grade solvents were eluted as steamed bread peaks, thus showing a gradually increasing baseline.
8.
Agilent compatible tips When using a guard column, if necessary, between injections or during the analysis, periodically flush the column with a strong solvent. A solvent cycle was used, but the detector was not adjusted.
9. Rebaseline. Use a new mobile phase when the detector dynamic range is changed.
10. The detector is not set at the wavelength of maximum absorption. Adjust the wavelength to the wavelength of maximum absorption.
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