PCR tube

PCR tubes are commonly used consumables in biological experiments. For example, BBSP PCR tubes are mainly used to provide containers for PCR (polymerase chain reaction) experiments, which can be applied to mutation, sequencing, methylation, molecular cloning, gene expression, Genotyping, medicine, forensic science and other fields. A common PCR tube is composed of a tube body and a cover, and the tube body and the cover are connected together.

The earliest PCR instrument did not have a hot cover. During the PCR process, the liquid at the bottom of the tube would evaporate to the top. The convex cover (that is, the round top) was designed to facilitate the evaporation of the liquid to condense and flow down. However, the current PCR instrument is basically a hot cover type. The temperature at the top of the PCR cover is high and the temperature at the bottom is low. The liquid at the bottom is not easy to evaporate to the top, so most of them use flat covers.

Features of PCR tube: Material: It is improved with the unique formula of the highest grade medical grade polypropylene (MDPP), which makes the product achieve better thermal conductivity, rapid response and uniform thermal conductivity than similar products.

Mold: Use high-precision small-throughput molds to produce PCR consumables to ensure the uniformity and synchronization of plastic raw materials in the mold. So that each PCR product has the greatest similarity and uniformity.

Experiments have proved that among the key factors affecting the PCR reaction: PCR instrument, enzyme, magnesium ion, buffer, primer annealing temperature, etc., among them, the quality of PCR consumables and the temperature accuracy of PCR instrument are closely related to the experimental results. PCR tube and PCR plate Extremely thin tube walls and high uniformity are required. The thickness of the PCR tube wall will affect the heat transfer and heat transfer rate. The color, uniformity, and light transmittance of the tube body will directly affect the results of PCR, especially aPCR (quantitative PCR).

When selecting PCR tube materials, the heat transfer, optical permeability, tolerance, air tightness and other characteristics of the product are given priority. Better thermal conductivity, fast response and uniform thermal conductivity.